2019 Galápagos Marine Iguana Report

        Conservation of the endemic Galápagos Marine Iguana: investigating the emerging threat of marine plastic pollution 

Jen Jones – Galápagos Conservation Trust (GCT), University of Exeter (UoE)

Juan Pablo Muñoz-Pérez – UNC-Chapel Hill & Universidad San Francisco de Quito (USFQ) Galápagos Science Center and  University of the Sunshine Coast (USC)

Marine iguana (male in the breeding season) on the lookout in San Cristóbal © Juan Pablo Muñoz-Pérez
Marine iguana (male in the breeding season) on the lookout in San Cristóbal © Juan Pablo Muñoz-Pérez

Taking health stats and key measurements of a marine iguana at La Lobería colony, San Cristóbal, Galápagos © Juan Pablo Muñoz-Pérez
Marine Iguana at La Lobería colony, San Cristóbal, Galápagos © Juan Pablo Muñoz-Pérez
Jen Jones and Cathy Hobbs teaching local volunteer Emanuel Revelo beach microplastic survey techniques in Cabo Douglas, Fernandina island © Greg Lewbart,
Jen Jones and Cathy Hobbs teaching local volunteer Emanuel Revelo beach microplastic survey techniques in Cabo Douglas, Fernandina island © Greg Lewbart,

Outcomes and Impact

  1. Across our ten sites, we have a strong dataset on the abundance and type of large plastic items in coastal zones that may prove an entanglement risk using standardised beach survey methods that have been co-developed with Park Rangers and our wider research network. Microplastic contamination will be profiled in beach sediments, the sea surface and benthic sediments to get a holistic picture of pollution and therefore exposure risk for different iguana populations. This information will allow us to highlight areas of high pollution to focus conservation attention and reduce risks to iguanas and other marine wildlife. 
A polluted site on San Cristóbal island (Caleta del Parque) were we sampled marine iguana scats © Juan Pablo Muñoz-Pérez,
A polluted site on San Cristóbal island (Caleta del Parque) were we sampled marine iguana scats © Juan Pablo Muñoz-Pérez,

2. At all sites we undertook a habitat survey to assess food availability for marine iguanas. Any plastic interactions with marine algae were recorded e.g. algae growing on plastic debris or plastic fishing lines entangled in marine algae. Samples were taken of green algae for microplastics and heavy metals analysis.

3. All protocols have been documented and have been shared with the Galápagos National Park Directorate. Two rangers have received training during fieldwork, and we have also engaged two further Park rangers with a plastics workshop in Quito in September 2019 where the project was discussed with a group of 35 scientists and managers from the Eastern Pacific region. We also presented some results at the Galápagos Day event in October 2019 in London at the Royal Geographical Society: https://Galápagosconservation.org.uk/Galápagos-day-2019-our-biggest-yet/

4. Following laboratory analysis of the samples, the resulting hotspot risk map and recommendations for ongoing monitoring of the risk of pollution to marine iguanas will be presented to the Galápagos National Park Directorate for incorporation into their Galápagos Marine Litter Management Plan (of which GCT is a strategic advisor), ensuring a long-lasting legacy for the project.

5. We have developed an educational case study resource for GCT’s Discovering Galápagos website for schools which is currently being redesigned for a relaunch in autumn 2020. This will complement existing marine iguana themed resources that are linked to the school curriculum written with fellow researcher, Dr Amy MacLeod. Sixteen families and more than 50 people attended two community events held at the GSC in San Cristóbal to explain the ongoing research and its importance. The attendees took part in different educational activities, among which are significant commitments to protect marine iguanas.

6. The detailed marine iguana health assessment included: Standard measurements, Blood films, Body temperature, Heart rate, Respiratory rate, iSTAT blood values (Chem8), Lactate, Blood spot cards (heavy metals, PCB’s, phthalates), Total bilirubin, Cholesterol, Alanine aminotransferase, Alkaline phosphatase, Albumin, Uric acid, Globulin and Aspartate transaminase. A preliminary assessment of the data indicates that the animals captured and sampled were clinically healthy based on standard vital signs, morphometrics, and blood value data. These values have been compared to published and unpublished data on the marine iguana.  The blood spot cards (used to detect toxins and heavy metals) have not yet been analyzed. No abnormalities were found during physical examination. This was a healthy group of mixed age and sex Marine Iguanas. 

  

Marine Iguana Amblyrhynchus cristatus
Marine Iguana Amblyrhynchus cristatus from the Cabo Douglas colony, Fernandina island, Galápagos © Jen Jones
Temporary field camp/ marine iguana sample station on the volcanic shoreline of Punta Moreno, western Isabela island with the awesome Wolf Volcano in the backdrop © Greg Lewbart
Temporary field camp/ marine iguana sample station on the volcanic shoreline of Punta Moreno, western Isabela island with the awesome Wolf Volcano in the backdrop © Greg Lewbart
Field Assistant Cathy Hobbs recording iguana measurements whilst the snorkel team collect habitat data in eastern Fernandina island © Juan Pablo Muñoz-Pérez

Data Analysis 

Juan Pablo undertook a research residency at the University of Exeter, UK in November 2019 to process the iguana scat samples to determine evidence of microplastic ingestion.  He also analysed all suspected particles by FT-IR (fourier transform infra-red spectroscopy) increasing the confidence in results

In total, 101 synthetic fibers and fragments were found across the scat samples from 98 animals. Each Marine Iguana scat had 2 – 4 synthetic particles per sample after controls were applied.

  • The most common type of synthetic particles found were:
  • Alpha- cellulose (99.5% pure) – likely cotton e.g. from clothing
  • Nylon – likely from fishing equipment
  • Polyester – likely from clothing, possibly fishing gear
  • Polyethylene – likely from degraded beach litter
A Marine Iguana Scat© Juan Pablo Muñoz-Pérez
A Marine Iguana Scat© Juan Pablo Muñoz-Pérez

Future 

The analysis of the remaining samples will be undertaken when laboratories reopen. We are hopeful that even with the delays from COVID-19, that all samples will be processed and the data analysed by September 2020. We are aiming for a journal submission by December 2020 presenting the results of this project kindly funded by the International Iguana Foundation. This work will continue to have impact as we work to undertake similar risk assessments with other species including the Galápagos green sea turtle and the Galápagos sea lion.